In 1752, scientist Rene Reamur first observed the action of digestive juices on food. In 1825, the scientist Berzelus observed that some substances produced by cells act as organic catalysts. Diastase enzyme was first discovered by French chemists Persoz and Payen in 1833. German physiologist Wilhelm Kuhne first used the term enzyme in 1878. In 1897, German scientist Edward Buchner identified zymase enzyme from sugar. For this he won the Nobel Prize in 1907. In 1926 scientist James B. Sumner) discovered urease enzyme from plant cells. Enzymes, he noted, are made up of proteins. Scientist John H. Northrop (1930-1935) crystallized pepsin, trypsin and chymotrypsin. Scientist Wendell M. Stanley (1930-1935) isolated the TMV protein.