1. Setting up a laboratory is expensive.
2. Necessary machinery and chemicals are scarce.
3. Lack of skilled and educated manpower.
4. Plant tissue in the laboratory may be infected by pathogens.
5. Non-sale of produced seedlings.
6. Infected seedlings due to lack of proper maintenance.
7. No new variation is created.
8. Transplantation is difficult as the produced seedlings are small.
9. The value of seedlings produced in the first stage is very high.
10. Delayed fruiting.