The tissue at the tip of the plant tip is called the meristem. The meristem is always sterile. Germ-free new seedlings are created by meristem culture through tissue culture technology. These germ-free plants do not get diseased. No need to apply pesticides and fungicides to plants. As a result, production costs are reduced and environmental pollution is prevented. In 1952, Morrell and Martin were the first to produce germ-free seedlings by culturing dahlia plant meristems. Currently, pineapple and tomato seedlings have been produced using this method.