The word Protoplast is formed from the Greek word Proto meaning origin and plasma meaning organization or matter. Protoplast means primitive matter. All parts of the cell except the cell wall are called protoplasts. German botanist Robert von Hanstein first used the term protoplast in 1880. According to I. K. Vasil (1980), the protoplast is the inner part of the cell wall that can be separated from the cell wall mechanically or by enzymes. Protoplasts that are produced artificially are called isolated protoplasts. Protoplast consists of two parts. Protoplasm and non-living matter.