Identification of dead and mutilated individuals by genome sequencing

1. Collection of individual samples: Samples are collected for identification of dead and mutilated individuals. Samples are collected from person’s blood drop, hair, teeth, nails, semen or semen, body parts etc.
2. Increasing the amount of sample: The amount of sample should be collected from the person’s body. If the amount of sample collected is too low, the amount is amplified in the PCR process.
3. Sequencing of a person’s sample: In the process of genome sequencing from a person’s sample, the adenine, guanine, cytosine and thymine bases of the DNA molecule are identified.
4. Sequencing of a person’s relatives: The adenine, guanine, cytosine and thymine bases of the DNA molecule of the person’s mother, father, brother, sister, son or daughter are identified.
5. Identification of the dead: matching the DNA sequence of the person with the sequence of relatives. Individuals are identified by matching DNA sequences. In this way, the identification of the deceased is done by identifying or matching the genome sequencing.

Criminal identification process by genome sequencing

1. Collection of samples: Blood drops, hair, teeth, nails, semen or semen, body parts etc. are collected from crime scene.
2. Increasing the quantity of samples: For the identification of criminals, the quantity of samples should be collected from the crime scene. If the amount of sample collected is too low, the amount is amplified in the PCR process.
3. Sequencing of samples: Adenine, guanine, cytosine and thymine bases of DNA molecules are identified in the process of genome sequencing from the obtained samples.
4. Suspect sequencing: Adenine, guanine, cytosine and thymine bases in DNA molecules of crime suspects are identified.
5. Criminal identification: The sequence of the sample DNA is matched to the sequence of the suspect. Criminals are identified by matching DNA sequences.

Genome sequencing in early characterization and disease diagnosis

Sequencing of samples from the embryo gives an idea about the baby’s future. How the child will behave, how it will grow, how tall it will be, how strong it will be etc. Genome sequencing of fetuses or newborns can lead to early diagnosis and preventive measures.

Human Genome Project Genome Sequencing

Genome sequencing allows the identification of the bases adenine, guanine, cytosine and thymine in DNA molecules. Genome sequencing technology has made it possible to determine the structure and function of the three billion nucleotide molecules of the 20,000-25,000 active genes on the 24 human chromosomes. This work took about 13 years (1990-2003).

Genome sequencing in agriculture

(i) Enhancement of plant resistance: Food producing plants are enhanced in resistance against harmful insects and pests.
(ii) Quality improvement: Increasing the quantity and quality of cattle meat, milk, eggs.
(iii) Diagnosis of Plant Diseases: Diagnosing and prevention of various plant diseases is being done. Disease diagnosis and prevention of plants like paddy, wheat, sugarcane, bhutra, mango, yam, jackfruit, litchi etc. have been provided.
(iv) Stress resistance: Genes for plant disease resistance, pest resistance and survival in adverse environments are being searched for. E.g. Bt toxin gene CrylAC and saline tolerance gene CPDH 45.
(v) Generation of new traits: Searching for genes with improved traits and successfully using them for improving plant quality.
(vi) Breeding in wild animals: DNA sequencing techniques are being applied in breeding wild animals like tigers, lions, elephants etc.
(vii) Unveiling the life secret of jute: Bangladeshi scientist Dr. Maqsudul Alam and his colleagues have uncovered the secret of jute’s life by sequencing the genome of Tosha jute (Corchorus olitorius). 120 crores of base pairs of jute. The order in which they are arranged is known. As a result, it will be possible to develop fine fiber jute, strong fiber jute like cotton, winter jute, medicinal jute, easily degradable jute, insect repellent jute etc.
(viii) RNAi method: Genome sequencing of Mugdal yellow mosaic virus has been done in Bangladesh. Disease resistant cultivars have been developed using RNAi methods.
(ix) Disease resistant tomato: Genome sequencing of ToLCV virus causing leaf curl disease of tomato has been done. Disease resistant cultivars have been developed using the ToLCV method.

DNA Forensic Genome Sequencing

(i) Paternity determination: Genome sequencing identifies the father or mother of the fetus. A child inherits half of its chromosomes from its parents. So the child’s DNA sequence reveals intermediate characteristics. In this way the paternity of the child can be determined.
(ii) Criminal Identification: Criminals involved in murder or rape can be identified through application of genome sequencing. Blood drops, hair, teeth, nails, semen or semen, body parts, etc. are collected from the crime scene. If the amount of sample obtained is too low, the amount is amplified in the PCR process. The genome sequencing of these samples is compared with the genome sequencing of the suspect. In this way the culprit can be identified.
(iii) Identification of corpses: Mutilated corpses can be identified by genome sequencing.
(iv) Identification of individuals: By examining the genome sequence, an idea about the physical structure, characteristics, color etc. of an individual can be obtained.
(v) Prediction of the child: Genome sequencing by taking samples from the embryo before the child is born can give an idea about the future of the child. The child is tall or short, black or fair, weak or strong, and the mannerisms are perceived.
(vi) Identification of Biodiversity: Genome sequencing is applied to identify the genetic diversity of plants.

Genome sequencing in medicine

(i) Transplantation of Organs: In case of transplantation of heart, lung, kidney, eye etc. from one body to another, it is necessary to know whether there is any adverse effect on donor or recipient. It is determined by DNA sequencing.
(ii) Prevention of side effects of drugs: Drugs cause various side effects in the human body. Genome sequencing can prevent drug side effects.
(iii) Preparation of medicine: In this method medicine can be prepared for specific diseases.
(iv) Gene therapy: Gene therapy can be given through this method. Gene therapy is a modern medical method.
(v) Detection of defective genes: Defective genes are harmful to the organism. In this method the sequenced gene is identified. After this, the sequenced gene is removed.
(vi) Cure of hereditary diseases: Through genome sequencing, causes of hereditary diseases like hemophilia, meiotic dystrophy, fragile X syndrome, neurofibromatosis-II etc. can be known and preventive measures can be taken.
(vii) Advance treatment: In this process the problems of various diseases can be known in advance and early treatment of diseases can be taken.
(viii) Cancer cure: Genome sequencing has successful application in research and treatment of cancer diseases. Genome sequencing is applied in cancer research such as colon cancer, breast cancer, somatic variation detection, transcriptome sequencing, virus tumor detection etc. Various topics of cancer research are being presented to researchers through bioinformatics.
(ix) Disease diagnosis: Genome sequencing is applied in some disease diagnosis. For example, Down’s syndrome.
(x) Treatment in short time: Accurate diagnosis is being made by applying genome sequencing. Extensive research is going on for proper treatment in less time and less cost.
(xi) Diagnosis of immune system: Reasons for susceptibility of people to disease and lack of immunity can be determined.
(xii) Alteration of DNA sequence: Direct application of various X-rays changes the DNA sequence.
(xiii) Control of Dengue: Genome sequencing technology has made control of the Aedes mosquito, the vector of dengue, possible. Researchers at the Oxitech company have created a breed of mosquitoes by altering genes. Their male mosquitoes cannot produce viable offspring. The larvae die soon after hatching. In this way, it has been possible to reduce 90% of mosquitoes in some cities in Brazil.