ড. মোঃ আবু বকর সিদ্দিক

বিএসসি-অনার্স (প্রথম শ্রেণী), এমএসসি (প্রথম শ্রেণী), এম. ফিল, পিএইচ ডি

(রাজশাহী বিশ্ববিদ্যালয়, বাংলাদেশ)

শ্রেষ্ঠ শিক্ষক ও স্বর্ণপদক-২০১১, ২০১২ এবং ২০১৩

লেখক, গবেষক ও শিক্ষাবিদ

বীরশ্রেষ্ঠ মুন্সী আব্দুর রউফ পাবলিক কলেজ

বিজিবি সদর দপ্তর

পিলখানা

ঢাকা

বাংলাদেশ

ড. সিদ্দিক পাবলিকেশন্স

গ্রন্থসমুহঃ এসএসসি, এইচএসসি, মেডিকেল ভর্তি পরীক্ষা, বিশ্ববিদ্যালয় ভর্তি পরীক্ষা এবং বিসিএস

বই সংগ্রহের জন্য যোগাযোগঃ WhatsApp 01511483701

 

Fingerprints, marks or marks on human hands are called tip signatures or finger prints. Identification of specific bands from highly variable regions of DNA with radioactive probes is called DNA fingerprinting. The complex process by which an individual can be distinguished from other individuals in genetic information by determining the arrangement of deoxyribonucleotides in DNA is called DNA fingerprinting. Due to variations in DNA (ATGC), every person in the world has a different fingerprint. Fingerprints are required for land registry, marriage certificate registry, biometric seam registration, school attendance confirmation, office staff attendance confirmation, any contract etc.

 

Discovery of DNA finger print

In 1985 scientist Alec Jeffreys discovered the finger printing method. In 1986 this method was adopted separately.

 

Collection of samples for DNA fingerprinting

Blood cells (WBC), semen, bone marrow, hair follicles, skin, nails, sputum, saliva, body parts etc. are collected as samples. These cells act as a source of DNA fingerprinting. DNA finger printing requires 1 microgram of banana.

 

DNA finger print method

Making a photographic pattern of an organism’s DNA in the process of gel electrophoresis is called a DNA finger print or DNA profile. DNA is collected and fragments are cut by restriction enzymes. In the gel electrophoresis process, particles are run over a gel layer. The cysts become progressively smaller and accumulate as aligned bands. Photographic patterns or impressions are obtained from the bands. It is called DNA fingerprint.

 

Importance of DNA Finger Print

1. Any criminal can be reliably identified through DNA fingerprinting.
2. DNA finger printing is very effective in identifying murderers, rapists, robbers etc.
3. Paternity of the child is determined by DNA finger printing.
4. Relationships between close relatives are determined through DNA fingerprinting.
5. Genetic databanks and land registries are done with this method.

 

DNA fingerprinting is better than dermal fingerprinting

Individuals and criminals can be identified by cutaneous fingerprinting or dermatoglyphics. Skin fingerprinting can be changed through plastic surgery. So this method is systematic. But DNA fingerprinting cannot be changed in any way. The DNA fingerprint of any cell, organ or organ in a person’s body is always the same. So DNA finger printing is best in criminal identification.

Individuals and criminals can be identified by cutaneous fingerprinting or dermatoglyphics. Skin fingerprinting can be changed through plastic surgery. So this method is systematic. But DNA fingerprinting cannot be changed in any way. The DNA fingerprint of any cell, organ or organ in a person’s body is always the same. So DNA finger printing is best in criminal identification.

1. Any criminal can be reliably identified through DNA fingerprinting.
2. DNA finger printing is very effective in identifying murderers, rapists, robbers etc.
3. Paternity of the child is determined by DNA finger printing.
4. Relationships between close relatives are determined through DNA fingerprinting.
5. Genetic databanks and land registries are done with this method.

1. Bam HI
2. Hind III
3. Hpa I
4. Eco RI
5. Eco RII
6. Sma I

Making a photographic pattern of an organism’s DNA in the process of gel electrophoresis is called a DNA finger print or DNA profile. DNA is collected and fragments are cut by restriction enzymes. In the gel electrophoresis process, particles are run over a gel layer. The cysts become progressively smaller and accumulate as aligned bands. Photographic patterns or impressions are obtained from the bands. It is called DNA fingerprint.

Blood cells (WBC), semen, bone marrow, hair follicles, skin, nails, sputum, saliva, body parts etc. are collected as samples. These cells act as a source of DNA fingerprinting. DNA finger printing requires 1 microgram of banana.

In 1985 scientist Alec Jeffreys discovered the finger printing method. In 1986 this method was adopted separately.

Fingerprints, marks or marks on human hands are called tip signatures or finger prints. Identification of specific bands from highly variable regions of DNA with radioactive probes is called DNA fingerprinting. The complex process by which an individual can be distinguished from other individuals in genetic information by determining the arrangement of deoxyribonucleotides in DNA is called DNA fingerprinting. Due to variations in DNA (ATGC), every person in the world has a different fingerprint. Fingerprints are required for land registry, marriage certificate registry, biometric seam registration, school attendance confirmation, office staff attendance confirmation, any contract etc.

Polymerase Chain Reaction is abbreviated as PCR. PCR is the fastest cloning technique. When the DNA vector is isolated and introduced into the host cell, the desired gene is spliced ​​with the host and numerous copies are made. In 1984, American scientist Kary Mullis invented the PCR technique.
Step-1: First the desired piece of DNA is heated to 95 degrees C. It separates the DNA strands. As a result, the primers bind to the designated sites. This step is called Denaturing step.
Step-2: Very quickly the temperature of the process is brought down. Primers bind to DNA primers. However, the actual temperature may be lower or higher depending on the length and sequence of the primer. A reaction that occurs once is called a cycle.
Step-3: At this stage, the reaction temperature is 45 degrees Celsius. is kept at (dNTPs) In the presence of Mg2+ ions, nucleotides link together to form new complementary DNA strands.
Step-4: The resulting DNA molecules are separated again by heat. Primer-1 binds to the 5→3 primer and Primer-2 binds to the 3→5 primer. At the end of the second cycle, the two progenitors make two new DNAs. The number of DNA molecules in each cycle will double that of the previous cycle.
With a machine, the reactions that make DNA go one after the other. This device is called thermal cycler. Each step takes 2-5 minutes. The reaction is limited to 25-35 cycles. One molecule of DNA will produce 235(2n) = 3.5×1010 molecules of DNA after 35 cycles.