1. The rate of glycolysis increases when the use of ATP is faster. The rate of this process slows down when the use of ATP is reduced.
2. The amount and supply of glucose regulates this process.
3. Allosteric enzyme phosphofructokinase which catalyzes the formation of fructose 1, 6-bisphosphate from fructose 6-phosphate. Glycolysis process is dependent on its speed.