The protein that is formed by wet analysis of protein is called secondary produced protein. Secondary produced proteins are:
(i) Protease: Protease is formed by the action of pepsin and trypsin. It is insoluble in water. But stored in heat. Albuline produces albumose and globulin produces globulose.
(ii) Peptone: Peptone is formed by the action of mild acid and enzymes. It is insoluble in water. Does not coagulate in heat. Ammonium sulfate does not precipitate in solution.
(iii) Peptides: Peptides consist of a small number of amino acids. Amino acids join together to form dipeptides, tripeptides and polypeptides. It is soluble in water. For example, glycine-alanine, leucine-glutamic acid.
(iv) Infraproteins: These are metalloproteins. eg metaprotein.
(v) Coagulated protein: This protein is produced when blood clots.