The cavity of the signet ring disappears within 8 hours. The parasite is irregular in shape, amoeba-like, and motile. This stage is called amoeboid trophozoite. Amoeboid trophozoites consume the protein component of hemoglobin as food. Hematin of hemoglobin is converted to toxic hemogene. At this time the red blood cell is enlarged in size and tiny granules are seen in its cytoplasm. These grains are called Schiffner’s dots. Malaria is diagnosed by the presence of softeners granules in blood cells. Schiffner’s dots are named after the inventor Wilhelm Schiffner (1904).