Biofuel or Biodiesel is made from algae. So algae are called second generation biofuels. Botryococcus braunii algae is being used to make biofuel. Attempts are being made to produce biofuel from Chlorella and Scenedesmus algae. Recently, researchers are trying to produce third generation biofuels from microalgal biomass. Some micro-algal species Kirchneriella lunaris, Ankistrodesmus fusiformis, Ankistrodesmus falcatus, Chlamydocapsa bacillus, Chlorella, Scenedesmus etc. have been taken for biodiesel production.