After placing the explants in the culture medium, the container is kept in a controlled environment. Room temperature is kept at 17-20°C, light intensity 3,000-5,000 lux or 1000-3000 lux and relative humidity 70-75%. Within a few days, the cell divides repeatedly to form a multicellular nucleus. This multicellular body is called a callus. After 5-7 days numerous buds or microflora are produced in the callus. Buds grow due to totipotency of cells. New seedlings are then produced from the buds.