Jaundice and fatigue symptoms can diagnose hepatitis. HBsAg surface antigen (HBsAg) is tested in blood to diagnose hepatitis-B. HBs antigen appears in the blood 6-12 weeks after infection. A blood test is done to diagnose Hepatitis-C. Anti-HCV is seen in the blood within 8-9 weeks of hepatitis-C infection. HCV RNA test is done if the blood is Anti-HCV positive. A positive HCV RNA test will diagnose hepatitis-C. Liver biopsy is sometimes required.