The stage after synthesis phase is called pause-2. The features of Biram-2 are-
(i) Cell metabolism increases in this condition.
(ii) Histone proteins, non-histone proteins, tRNA, mRNA, rRNA etc. are synthesized.
(iii) Nucleus increases in volume as various elements are prepared.
(iv) In this phase the damaged DNA molecule is repaired.
(v) Two centrosomes arise from one centrosome. Centrosomes form microtubules. Microtubules form spindle fibers.
(vi) Cells enter mitosis from G2 phase with the help of maturation promoting factor (MPF).
(vii) Volume of nucleus and cytoplasm increases.
(viii) This sub-phase takes 10-20% time.