Two methods of genome sequencing are – Chemical degradation method and Chain termination method. In 1976 A. Maxam I W. Gilbert Chemical degradation method and in 1977 F. Sanger I A.R. Coulsor invented the chain termination method. Genome sequencing process is described as-
1. DNA molecules are divided into 4 test tubes enriched with reagents.
2. Each of the four reactions is separated by gel electrophoresis.
3. Genome sequencing is determined from the location and size of radioactive bands.
4. The results of electrophoresis are analyzed and interpreted using a computer aided X-ray scanner.