(i) Transgenic cotton plants have been created by introducing a gene from Bacillus bacteria into American cotton plants. These transgenic cotton plants produce a toxic protein. Due to this, insect attack does not occur. As a result cotton production increased. Along with this, production costs are reduced and environmental pollution is prevented.
(ii) At present insect resistant plants like potato, apple, cotton, wheat etc. have been developed. Bt toxin gene and CpTi gene have been inserted in these plants. Insects die within 24 hours after eating the leaves of these plants.
(iii) USA company has produced caterpillar resistant cotton seeds. As a result, pest problems in cotton cultivation have been eliminated.
(iv) Cry gene isolated from Bacillus thuringiensis bacteria and introduced into plants to create insect resistant plants. Transgenic plants like Bt brinjal, Bt cotton, Bt rice, Bt corn, Bt potato, Bt apple are resistant to pests.
(v) Larvae attack of European cornbora moth causes extensive damage to bhutra and yield reduction of 40%. Transgenic bhutra has been created by introducing genes from bacteria that produce toxic proteins into bhutra plants. This bhutra is not affected by cornbora. Now there is no need to apply pesticides in Bhutra cultivation. This has increased the yield of Bhutra, reduced production costs and prevented environmental pollution.
(vi) Rhyzobium was introduced into Rhyzobium with toxin producing gene from Bacillus thuringiensis bacteria. Rhyzobium causes nodules on the roots of legumes. Bean plants with nodules can prevent attacks by harmful insects like weevils.
(vii) Bt toxin gene has been created by introducing the gene from Bacillus thuringiensis bacteria into cotton plant. Insects such as Lepidoptera, beetles, flies, mosquitoes etc. have died by eating cotton plants containing Bt toxin gene.
(viii) Sterile Insect Technique (SIT) is a modern method. It is an environmentally friendly pest control method. Males of harmful insects are sterilized by this method. As a result the new generation does not develop. This technology is being used in countries like Brazil, Japan, Philippines, Thailand, USA etc.