The first and most important step in tissue culture technology is explant selection and isolation. First the explants are selected. While selecting the explant, it should be kept in mind that it should be healthy, vigorous, disease free and of improved variety. Besides, care is taken to ensure that the desired features are present in it. Later, the required parts such as apical bud, lateral bud or leaf part, dividing cells, meristem, pollen, ovule, embryo, nucellus, protoplast etc. are separated from the explant.