Another important step in tissue culture technology is the preparation of culture media. Usually this culture media is made up of essential plant nutrients. The culture media is prepared with the main and secondary plant nutrients, vitamins, phytohormones, agar agar, glucose, sucrose (2-4%), fructose, maltose etc. in a container. This culture medium prepared with basic nutrients is called basal medium. The pH of the growing medium is kept between 5.5-6.0. The most commonly used culture media are MS medium (Murashige & Skoog, 1962) and B5 (Gamborg et al, 1968) medium.