Gene engineering in interferon production

Interferon is a high molecular weight protein. It inhibits the growth of cancer cells and inactivates viruses. Interferon is produced from E. coli and yeast through genetic engineering. It is used in the treatment of hepatitis-B, herpes, papilloma, rabies etc. Interferon was discovered in 1957 by Alec Isaacs and Jean Lindenman.

Recombinant DNA in the treatment of hereditary diseases

Hereditary diseases are difficult to cure. Antithrombin-III from transgenic goat milk and III C12 esterase inhibitor drugs from transgenic rabbit milk have been developed by recombinant DNA technology. Antithrombin-III drugs are used in the treatment of human antithrombin deficiency and III C12 esterase inhibitor drugs in the treatment of human angioedema. Efforts are underway to eradicate hereditary diseases by discovering more drugs through this technology. Each human cell contains up to 25,000 (many more are unknown) functional genes. A disorder in one of the genes can cause the disease. About 3500 genetic disorders are known in human body. This disease can be cured by recombinant DNA technology.

Genetic Engineering for Vaccines

Gene engineering technology is used to produce vaccines or vaccines for various diseases. These vaccines are used to treat infectious diseases like Measles, Polio, Smallpox, Tuberculosis, Rabies, Cholera, Mumps, Hepatitis-B etc. At present, efforts are being made to make effective vaccines for various diseases including corona. It will be possible to ensure the protection of human health by discovering new vaccines.

Role of recombinant DNA in stress resistant cultivars

(i) Pyrroline-5 Carboxylase Synthase gene isolated from soybean and inserted into tobacco plant was found to grow ten times more in saline soil.
(ii) Cold tolerant AF gene isolated from arctic fish (Flounder) and introduced into crop plants to create cold tolerant crops. For example, tomatoes, round potatoes etc.
(iii) Drought resistant Bhutra has been developed by introducing csPB gene from Bacillus subtilis bacterium into Bhutra plant.
(iv) Introgression of the At NHXI gene from Arabidopsis into peanut plants has resulted in salted peanuts.
(v) Genes resistant to stresses such as heat, cold, heavy metals, phytohormones etc. have been identified in natural environment. All these genes are trying to enter the plant body.